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Journal article

Direct observation of end resection by RecBCD during double-stranded DNA break repair in vivo

Abstract:

The formation of 3′ single-stranded DNA overhangs is a first and essential step during homology-directed repair of double-stranded breaks (DSB) of DNA, a task that in Escherichia coli is performed by RecBCD. While this protein complex has been well characterized through in vitro single-molecule studies, it has remained elusive how end resection proceeds in the crowded and complex environment in live cells. Here, we develop a two-color fluorescent reporter to directly observe the resection of ...

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Publication status:
Published
Peer review status:
Peer reviewed
Version:
Publisher's Version

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Publisher copy:
10.1093/nar/gkx1290

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Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
Biochemistry
Role:
Author
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Funding agency for:
Sherratt, DJ
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Funding agency for:
Sherratt, DJ
Publisher:
Oxford University Press Publisher's website
Journal:
Nucleic Acids Research Journal website
Volume:
46
Issue:
4
Pages:
1821-1833
Publication date:
2017-12-23
Acceptance date:
2017-12-18
DOI:
EISSN:
1362-4962
ISSN:
0305-1048
Pubs id:
pubs:817807
URN:
uri:cbf13b2f-b656-423e-bccd-da81f7fd28d1
UUID:
uuid:cbf13b2f-b656-423e-bccd-da81f7fd28d1
Local pid:
pubs:817807
Language:
English
Keywords:

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