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Journal article

Protein-protein interaction site mapping using NMR-detected mutational scanning.

Abstract:
We demonstrate a novel NMR method for the mapping of protein-protein interaction sites. In our approach protein-protein binding sites are mapped by competition binding experiments using indirect NMR reporter technology and Ala positional scanning. The methodology provides high sensitivity, ease of implementation and high-throughput capabilities. The feasibility of the technique is demonstrated with an application to the beta-Catenin/Tcf4 complex.

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Journal:
Journal of biomolecular NMR
Volume:
38
Issue:
2
Pages:
133-137
Publication date:
2007-01-01
ISSN:
0925-2738

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