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The improved procedure for the expression, purification and crystallization of mutants of cytochrome P450cam and the crystal structure of the F87L/Y96F/L244A/V247A mutant

Abstract:

The UV absorbance ratio (A392/A280) was developed to survey the purity of the P450cam mutant protein during the purification of the mutants protein. Thus the course of the purification became compact and the efficiency of the purification had been improved. β-Mercaptoethenol had been developed to maintain the deoxidization of the protein during the purification. Crystals of the F87L/Y96F/L244A/V247A mutant were grown by vapor diffusion method. X-ray diffraction data were collected to 0.22 nm ...

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Publication status:
Published

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Journal:
PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
Volume:
31
Issue:
7
Pages:
611-615
Publication date:
2004-07-05
ISSN:
1000-3282
URN:
uuid:cbc52dff-09eb-4ad5-8e3f-4ccf2eff9770
Source identifiers:
39141
Local pid:
pubs:39141

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