Direct photoaffinity labeling of Kir6.2 by [gamma-(32)P]ATP-[gamma]4-azidoanilide.

Journal article

ATP-sensitive potassium (K(ATP)) channels are under complex regulation by intracellular ATP and ADP. The potentiatory effect of MgADP is conferred by the sulfonylurea receptor subunit of the channel, SUR, whereas the inhibitory effect of ATP appears to be mediated via the pore-forming subunit, Kir6.2. We have previously reported that Kir6.2 can be directly labeled by 8-azido-[gamma-(32)P]ATP. However, the binding affinity of 8-azido-ATP to Kir6.2 was low probably due to modification at 8' pos...

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Authors: Tanabe, K; Tucker, S; Ashcroft, F; Proks, P; Kioka, N

• Publication status: Published
• Journal: Biochemical and biophysical research communications
• Volume: 272
• Issue: 2
• Pages: 316-319
• Publication date: 2000-06-01
• DOI: 10.1006/bbrc.2000.2780
• EISSN: 1090-2104
• ISSN: 0006-291X
• Language: English
• Keywords: Azides; Potassium Channels, Inwardly Rectifying; Dose-Response Relationship, Drug; Potassium Channel Blockers; Xenopus laevis; ATP-Binding Cassette Transporters; Oocytes; Transfection; Recombinant Fusion Proteins; Potassium; Adenosine Triphosphate; Potassium Channels; Binding, Competitive; Magnesium; Substrate Specificity; Animals; Rats; Receptors, Drug; Sequence Deletion; Photoaffinity Labels; COS Cells; Electric Conductivity