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Fluorescence measurement of intracellular sodium concentration in single Escherichia coli cells.

Abstract:

The energy-transducing cytoplasmic membrane of bacteria contains pumps and antiports maintaining the membrane potential and ion gradients. We have developed a method for rapid, single-cell measurement of the internal sodium concentration ([Na(+)](in)) in Escherichia coli using the sodium ion fluorescence indicator, Sodium Green. The bacterial flagellar motor is a molecular machine that couples the transmembrane flow of ions, either protons (H(+)) or sodium ions (Na(+)), to flagellar rotation....

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Publication status:
Published

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Publisher copy:
10.1529/biophysj.105.071332

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Institution:
University of Oxford
Department:
Oxford, MPLS, Physics, REF-Physics
More by this author
Institution:
University of Oxford
Department:
Oxford, MPLS, Physics, Condensed Matter Physics
Journal:
Biophysical journal
Volume:
90
Issue:
1
Pages:
357-365
Publication date:
2006-01-05
DOI:
EISSN:
1542-0086
ISSN:
0006-3495
URN:
uuid:cb737c5d-a641-48f9-9d8a-2818f00391ea
Source identifiers:
17010
Local pid:
pubs:17010

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