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A method of isolating viable chondrocytes with proliferative capacity from cryopreserved human articular cartilage.

Abstract:

This study aimed to optimise methods of cryopreserving human articular cartilage (AC) tissue for the isolation of late chondrocytes. Human AC specimens from osteoarthritis patients who had undergone total knee replacement were used to optimise the chondrocyte isolation process and the choice of cryoprotective agent (CPA). For AC tissue cryopreservation, intact cored cartilage discs (5 mm diameter) and diced cartilage (0.2-1 mm cubes) from the same sized discs were step cooled and stored in li...

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Publication status:
Published

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Publisher copy:
10.1007/s10561-012-9328-y

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Institution:
University of Oxford
Department:
Oxford, MSD, NDORMS
Role:
Author
Journal:
Cell and tissue banking
Volume:
14
Issue:
2
Pages:
267-276
Publication date:
2013-06-05
DOI:
EISSN:
1573-6814
ISSN:
1389-9333
URN:
uuid:cb5e400c-b56f-4ad9-977c-9776a47e704e
Source identifiers:
342315
Local pid:
pubs:342315

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