- Abstract:
-
This study aimed to optimise methods of cryopreserving human articular cartilage (AC) tissue for the isolation of late chondrocytes. Human AC specimens from osteoarthritis patients who had undergone total knee replacement were used to optimise the chondrocyte isolation process and the choice of cryoprotective agent (CPA). For AC tissue cryopreservation, intact cored cartilage discs (5 mm diameter) and diced cartilage (0.2-1 mm cubes) from the same sized discs were step cooled and stored in li...
Expand abstract - Publication status:
- Published
- Publisher copy:
- 10.1007/s10561-012-9328-y
-
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- Journal:
- Cell and tissue banking
- Volume:
- 14
- Issue:
- 2
- Pages:
- 267-276
- Publication date:
- 2013-06-05
- DOI:
- EISSN:
-
1573-6814
- ISSN:
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1389-9333
- URN:
-
uuid:cb5e400c-b56f-4ad9-977c-9776a47e704e
- Source identifiers:
-
342315
- Local pid:
- pubs:342315
- Copyright date:
- 2013
Journal article
A method of isolating viable chondrocytes with proliferative capacity from cryopreserved human articular cartilage.
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