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Nanopore metagenomic sequencing for detection and characterization of SARS-CoV-2 in clinical samples

Abstract:
Objectives
The COVID-19 pandemic has underscored the need for rapid novel diagnostic strategies. Metagenomic Next-Generation Sequencing (mNGS) may allow for the detection of pathogens that can be missed in targeted assays. The goal of this study was to assess the performance of nanopore-based Sequence-Independent Single Primer Amplification (SISPA) for the detection and characterization of SARS-CoV-2.
Methods
We performed mNGS on clinical samples... Expand abstract
Publication status:
Published
Peer review status:
Peer reviewed

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Institution:
University of Oxford
Division:
MPLS
Department:
Zoology
Oxford college:
St Peter's College
Role:
Author
ORCID:
0000-0003-0250-0423
Publisher:
Public Library of Science
Journal:
PLoS One More from this journal
Volume:
16
Issue:
11
Article number:
e0259712
Publication date:
2021-11-18
Acceptance date:
2021-10-25
DOI:
EISSN:
1932-6203
Language:
English
Keywords:
Pubs id:
1205852
Local pid:
pubs:1205852
Deposit date:
2021-10-26

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