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Gastrobodies are engineered antibody mimetics resilient to pepsin and hydrochloric acid

Abstract:

Protein-based targeting reagents, such as antibodies and non-antibody scaffold proteins, are rapidly inactivated in the upper gastrointestinal (GI) tract. Hydrochloric acid in gastric juice denatures proteins and activates pepsin, concentrations of which reach 1 mg/mL in the mammalian stomach. Two stable scaffold proteins (nanobody and nanofitin), previously developed to be protease-resistant, were completely digested in less than 10 min at 100-fold lower concentration of pepsin than found in...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1038/s42003-021-02487-2

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Institution:
University of Oxford
Division:
MSD
Department:
Biochemistry
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MSD
Department:
Biochemistry
Role:
Author
Publisher:
Springer Nature
Journal:
Communications Biology More from this journal
Volume:
4
Issue:
2021
Article number:
960
Publication date:
2021-08-11
Acceptance date:
2021-06-18
DOI:
EISSN:
2399-3642
Language:
English
Keywords:
Pubs id:
1186251
Local pid:
pubs:1186251
Deposit date:
2021-07-14

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