Cloning of the HLA class II region in yeast artificial chromosomes.

Journal article

Yeast artificial chromosomes (YACs) have been applied to clone the entire class II region of the human major histocompatibility complex (MHC), including its flanking regions, in a contig over 1.5 million base pairs (bp) long. The human DNA inserts in the YACs have a size between 60 and 1300 kbp and were isolated from two EcoRI partial digest libraries. The gaps between DRA and DRB, DRB and DQA, and DOB and DPA, which had not been cloned by other means, have been bridged with YAC clones. The c...

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Authors: Ragoussis, J; Monaco, A; Mockridge, I; Kendall, E; Campbell, R

• Publication status: Published
• Journal: Proceedings of the National Academy of Sciences of the United States of America
• Volume: 88
• Issue: 9
• Pages: 3753-3757
• Publication date: 1991-05-01
• DOI: 10.1073/pnas.88.9.3753
• EISSN: 1091-6490
• ISSN: 0027-8424
• Language: English
• Keywords: Saccharomyces cerevisiae; Humans; Cosmids; Genetic Vectors; Blotting, Southern; Cloning, Molecular; Chromosomes; Histocompatibility Antigens; Haplotypes; Major Histocompatibility Complex; Chromosome Mapping; Restriction Mapping; HLA-D Antigens; Polymorphism, Restriction Fragment Length