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Journal article

Highly efficient targeted mutagenesis of Drosophila with the CRISPR/Cas9 system.

Abstract:

Here, we present a simple and highly efficient method for generating and detecting mutations of any gene in Drosophila melanogaster through the use of the CRISPR/Cas9 system (clustered regularly interspaced palindromic repeats/CRISPR-associated). We show that injection of RNA into the Drosophila embryo can induce highly efficient mutagenesis of desired target genes in up to 88% of injected flies. These mutations can be transmitted through the germline to make stable lines. Our system provides...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1016/j.celrep.2013.06.020

Authors


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Institution:
University of Oxford
Division:
MSD
Department:
Physiology Anatomy & Genetics
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MSD
Department:
Physiology Anatomy & Genetics
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MSD
Department:
Physiology Anatomy & Genetics
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MSD
Department:
Physiology Anatomy & Genetics
Role:
Author
Publisher:
Elsevier
Journal:
Cell reports More from this journal
Volume:
4
Issue:
1
Pages:
220-228
Publication date:
2013-07-01
DOI:
EISSN:
2211-1247
ISSN:
2211-1247
Language:
English
Keywords:
UUID:
uuid:ca48b2c0-e000-422b-9aca-d6856493eecb
Local pid:
pubs:411152
Source identifiers:
411152
Deposit date:
2013-11-16

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