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Isolation of cDNA clones encoding proteins of complex structures: analysis of the Trypanosoma brucei cytoskeleton.

Abstract:

We have adapted a group of well-known procedures in order to devise a simple method that allows the isolation of specific cDNAs encoding proteins located in different regions of the Trypanosoma brucei cytoskeleton. cDNA clones were isolated by screening a lambda gt11 expression library with a polyspecific, polyclonal antiserum against a complex immunogen, in this case the complete cytoskeleton. The fusion proteins produced by the clones were then used as an affinity immunoadsorbant to select ...

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Institution:
University of Oxford
Division:
MSD
Department:
Pathology Dunn School
Role:
Author
Journal:
Gene More from this journal
Volume:
110
Issue:
1
Pages:
65-70
Publication date:
1992-01-01
DOI:
EISSN:
1879-0038
ISSN:
0378-1119
Language:
English
Keywords:
Pubs id:
pubs:4782
UUID:
uuid:ca464acb-6ff3-4f82-a938-5d58d551914f
Local pid:
pubs:4782
Source identifiers:
4782
Deposit date:
2012-12-19

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