Isolation of cDNA clones encoding proteins of complex structures: analysis of the Trypanosoma brucei cytoskeleton.

Journal article

We have adapted a group of well-known procedures in order to devise a simple method that allows the isolation of specific cDNAs encoding proteins located in different regions of the Trypanosoma brucei cytoskeleton. cDNA clones were isolated by screening a lambda gt11 expression library with a polyspecific, polyclonal antiserum against a complex immunogen, in this case the complete cytoskeleton. The fusion proteins produced by the clones were then used as an affinity immunoadsorbant to select ...

Expand abstract

Authors: Birkett, C; Parma, A; Gerke-Bonet, R; Woodward, R; Gull, K

• Publication status: Published
• Journal: Gene
• Volume: 110
• Issue: 1
• Pages: 65-70
• Publication date: 1992-01-01
• DOI: 10.1016/0378-1119(92)90445-u
• EISSN: 1879-0038
• ISSN: 0378-1119
• Language: English
• Keywords: Immune Sera; Cloning, Molecular; Antibody Specificity; Macromolecular Substances; Antibodies, Protozoan; Protozoan Proteins; Animals; DNA, Protozoan; Cytoskeleton; Trypanosoma brucei brucei; Rabbits