Journal article

The Hepatitis C Virus E2p7 localization and topology in a recombinant system

Abstract:: The Hepatitis C virus (HCV) genome encodes a polyprotein that is processed co- and posttranslationally. Incomplete processing at the E2/p7 junction generates the E2p7 product. Using a recombinant system, we analysed the processing, localization and topology of E2p7. By immunoprecipitation of proteins expressed by metabolically labelled cells, we confirm that E2p7 is a precursor of E2. E2p7 forms a native-like heterodimer with E1, and it is localized entirely to the endoplasmic reticulum, in contrast to fully processed E2 and p7 that leak to the plasma membrane. No change in the topology of p7 was observed upon processing of E2p7, indicating that incomplete cleavage at the E2/p7 site is not regulated by changes in p7 membrane topology.

Publication status:: Published

Peer review status:: Peer reviewed

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APA Style

Popescu, C., Grigorescu, A., Dwek, R., & Zitzmann, N. (2009). The Hepatitis C Virus E2p7 localization and topology in a recombinant system. Romanian Journal of Biochemistry, 46(2), 149–163.

MLA Style

Popescu, C., et al. “The Hepatitis C Virus E2p7 Localization and Topology in a Recombinant System.” Romanian Journal of Biochemistry, vol. 46, no. 2, Editura Academiei Romane, 2009, pp. 149–63.

Chicago Style

Popescu, C, A Grigorescu, R Dwek, and N Zitzmann. 2009. “The Hepatitis C Virus E2p7 Localization and Topology in a Recombinant System.” Romanian Journal of Biochemistry 46 (2): 149–63.
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Authors

+ Popescu, C More by this author

Role:: Author

+ Grigorescu, A More by this author

Role:: Author

+ Dwek, R More by this author

Institution:: University of Oxford
Division:: MSD
Department:: Biochemistry
Role:: Author

+ Zitzmann, N More by this author

Institution:: University of Oxford
Division:: MSD
Department:: Biochemistry
Role:: Author

Publisher:: Editura Academiei Romane
Journal:: Romanian Journal of Biochemistry More from this journal
Volume:: 46
Issue:: 2
Pages:: 149-163
Publication date:: 2009-01-01
ISSN:: 1582-3318

Keywords:: polyprotein processing

topology

hepatitis C virus

E2p7 glycoprotein
Pubs id:: pubs:207601
UUID:: uuid:ca3e6dc4-abff-4780-8245-534311d74b24
Local pid:: pubs:207601
Source identifiers:: 207601
Deposit date:: 2016-02-05

Terms of use

Copyright holder:: Editura Academiei Romane
Copyright date:: 2009
Notes:: The full text of this article is not currently available in ORA but may be accessed on the publisher's website at http://journal.biochim.ro/archive/n46-2/abstracts46-2.php?abs=4 and http://journal.biochim.ro/archive/n46-2/pdfs_46-2/46-2_04.pdf

Licence:: Terms and Conditions of Use for Oxford University Research Archive

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