Can cytoplasmic nucleophosmin be detected by immunocytochemical staining of cell smears in acute myeloid leukemia?

Journal article

Mutations in the C-terminal region of nucleophosmin in acute myeloid leukemia (AML) result in aberrant cytoplasmic nucleophosmin (cNPM) in leukemic blast cells which is detectable by immunocytochemistry in bone marrow trephine (BMT) biopsy sections. We tested whether cNPM is detectable by immunocytochemistry in air-dried smears of AML with nucleophosmin1 (NPM1) mutations. An immunoalkaline phosphatase method was developed using the OCI-AML3 cell line, known to have mutated NPM1, and assessed ...

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Authors: Mattsson, G; Turner, S; Cordell, J; Ferguson, D; Schuh, A

• Publication status: Published
• Journal: Haematologica
• Volume: 95
• Issue: 4
• Pages: 670-673
• Publication date: 2010-04-01
• DOI: 10.3324/haematol.2009.011817
• EISSN: 1592-8721
• ISSN: 0390-6078
• Language: English
• Keywords: Bone Marrow; Mutation; Immunoenzyme Techniques; Cytoplasm; Prognosis; Polymerase Chain Reaction; Cytological Techniques; Humans; Leukemia, Myeloid, Acute; Nuclear Proteins