The dependence of DNase I activity on the conformation of oligodeoxynucleotides.

Journal article

We have developed a sensitive continuous assay for nucleases using proton release. The assay has been applied to the determination of the kinetics of DNase I acting on short, defined deoxyoligonucleotides. The dependence of Kcat/K(m) on sequence and structure of short oligonucleotide substrates has been measured: increasing lengths of AnTn sequences decrease the rate of cleavage. G.A mismatches in which the bases pair using imino protons are cleaved quite effectively by DNase I. In contrast, ...

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Authors: Sutton, D; Conn, G; Brown, T; Lane, A

• Publication status: Published
• Journal: Biochemical journal
• Volume: 321 ( Pt 2)
• Pages: 481-486
• Publication date: 1997-01-01
• EISSN: 1470-8728
• ISSN: 0264-6021
• Language: English
• Keywords: RNA; Deoxyribonuclease I; Base Sequence; Benzamidines; Cattle; Base Composition; DNA; Animals; Thymus Gland; Nucleic Acid Conformation; Oligodeoxyribonucleotides