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The dependence of DNase I activity on the conformation of oligodeoxynucleotides.

Abstract:

We have developed a sensitive continuous assay for nucleases using proton release. The assay has been applied to the determination of the kinetics of DNase I acting on short, defined deoxyoligonucleotides. The dependence of Kcat/K(m) on sequence and structure of short oligonucleotide substrates has been measured: increasing lengths of AnTn sequences decrease the rate of cleavage. G.A mismatches in which the bases pair using imino protons are cleaved quite effectively by DNase I. In contrast, ...

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Publication status:
Published

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Institution:
University of Oxford
Division:
MPLS
Department:
Chemistry
Sub department:
Organic Chemistry
Role:
Author
Journal:
Biochemical journal More from this journal
Volume:
321 ( Pt 2)
Pages:
481-486
Publication date:
1997-01-01
EISSN:
1470-8728
ISSN:
0264-6021
Language:
English
Keywords:
Pubs id:
pubs:400352
UUID:
uuid:ca1283ca-edfd-4b15-a0bc-42d0f2cfa385
Local pid:
pubs:400352
Source identifiers:
400352
Deposit date:
2013-11-16

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