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The role of arginine residues in substrate binding and catalysis by deacetoxycephalosporin C synthase.

Abstract:

Deacetoxycephalosporin C synthase (DAOCS) catalyses the oxidative ring expansion of penicillin N, the committed step in the biosynthesis of cephamycin C by Streptomyces clavuligerus. Site-directed mutagenesis was used to investigate the seven Arg residues for activity (74, 75, 160, 162, 266, 306 and 307), selected on the basis of the DAOCS crystal structure. Greater than 95% of activity was lost upon mutation of Arg-160 and Arg266 to glutamine or other residues. These results are consistent w...

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Publication status:
Published

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Institution:
University of Oxford
Division:
MPLS
Department:
Chemistry
Sub department:
Organic Chemistry
Role:
Author
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Journal:
European journal of biochemistry / FEBS
Volume:
269
Issue:
11
Pages:
2735-2739
Publication date:
2002-06-01
DOI:
EISSN:
1432-1033
ISSN:
0014-2956
Source identifiers:
32123
Language:
English
Keywords:
Pubs id:
pubs:32123
UUID:
uuid:ca074232-2fa5-43f6-a457-1706c4e4f4f4
Local pid:
pubs:32123
Deposit date:
2012-12-19

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