Journal article
Detecting protein aggregation and interactions in live cells: a guide to Number and Brightness
- Abstract:
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The possibility to detect and quantify protein-protein interactions with good spatial and temporal resolutions in live cells is crucial in biology. Number and Brightness is a powerful approach to detect both protein aggregation/desegregation dynamics and stoichiometry in live cells. Importantly, this technique can be applied in commercial set ups: both camera based and laser scanning microscopes. It provides pixel-by-pixel information on protein oligomeric states. If performed with two colour...
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- Publication status:
- Published
- Peer review status:
- Peer reviewed
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Access Document
- Files:
-
-
(Accepted manuscript, pdf, 1.1MB)
-
- Publisher copy:
- 10.1016/j.ymeth.2017.12.001
Authors
Funding
+ Wellcome Trust
More from this funder
Funding agency for:
Nolan, R
Grant:
105278/Z/14/2
203852/Z/16/2
Bibliographic Details
- Publisher:
- Elsevier Publisher's website
- Journal:
- Methods Journal website
- Volume:
- 140-141
- Pages:
- 172-177
- Publication date:
- 2017-12-06
- Acceptance date:
- 2017-12-03
- DOI:
- EISSN:
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1095-9130
- ISSN:
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1046-2023
Item Description
- Pubs id:
-
pubs:809874
- UUID:
-
uuid:c9ec3a6b-a705-4140-9bff-e72b9442cd3a
- Local pid:
- pubs:809874
- Source identifiers:
-
809874
- Deposit date:
- 2017-12-08
Terms of use
- Copyright holder:
- © 2017 Elsevier Inc All rights reserved This is the accepted manuscript version of the article The final version is available online from Elsevier at: 101016/jymeth201712001
- Copyright date:
- 2017
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