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Journal article

Biotagging, an in vivo biotinylation approach for cell-type specific subcellular profiling in zebrafish

Abstract:

Interrogation of gene regulatory circuits in complex organisms requires precise and robust methods to label cell-types for profiling of target proteins in a tissue-specific fashion as well as data analysis to understand interconnections within the circuits. There are several strategies for obtaining cell-type and subcellular specific genome-wide data. We have developed a methodology, termed “biotagging” that uses tissue-specific, genetically encoded components to biotinylate target proteins, ...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1016/j.ymeth.2018.07.011

Authors


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Role:
Author
ORCID:
0000-0002-2547-3694
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Division:
MSD
Department:
RDM
Sub department:
RDM Clinical Laboratory Sciences
Role:
Author
ORCID:
0000-0001-9289-0263
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Name:
Medical Research Council
Grant:
G0902418
Publisher:
Elsevier
Journal:
Methods More from this journal
Volume:
150
Pages:
24-31
Publication date:
2018-08-02
Acceptance date:
2018-07-30
DOI:
EISSN:
1095-9130
ISSN:
1046-2023
Pmid:
30076893
Language:
English
Keywords:
Pubs id:
908241
Local pid:
pubs:908241
Deposit date:
2020-06-09

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