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An in cellulo-derived structure of PAK4 in complex with its inhibitor Inka1

Abstract:

PAK4 is a metazoan-specific kinase acting downstream of Cdc42. Here we describe the structure of human PAK4 in complex with Inka1, a potent endogenous kinase inhibitor. Using single mammalian cells containing crystals 50 μm in length, we have determined the in cellulo crystal structure at 2.95 Å resolution, which reveals the details of how the PAK4 catalytic domain binds cellular ATP and the Inka1 inhibitor. The crystal lattice consists only of PAK4-PAK4 contacts, which form a hexagonal arra...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1038/ncomms9681

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Institution:
University of Oxford
Division:
MSD
Department:
NDM
Sub department:
Structural Biology
Role:
Author
Publisher:
Nature Publishing Group Publisher's website
Journal:
Nature Communications Journal website
Volume:
6
Pages:
8681
Publication date:
2015-11-26
Acceptance date:
2015-09-21
DOI:
EISSN:
2041-1723
Language:
English
Keywords:
Pubs id:
pubs:579321
UUID:
uuid:c9acf3cb-8e0d-4c98-967f-4eeb32ccb933
Local pid:
pubs:579321
Source identifiers:
579321
Deposit date:
2016-07-14

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