Selective deactivation of serum IgG: A general strategy for the enhancement of monoclonal antibody receptor interactions

Journal article

Serum IgG is a potent inhibitor of monoclonal antibody (mAb) binding to the cell-surface Fcγ receptors (FcγRs), which mediate cytotoxic and phagocytic effector functions. Here, we show that this competition can be eliminated, selectively, by the introduction to serum of (i) an enzyme that displaces Fc from FcγRs and (ii) a modification present in the therapeutic mAb that renders it resistant to that enzyme. Specifically, we show that (i) EndoS (endoglycosidase S) cleaves only complex-type gly...

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Authors: Baruah, K; Bowden, T; Krishna, B; Dwek, R; Crispin, M

• Journal: Journal of Molecular Biology
• Volume: 420
• Issue: 1-2
• Pages: 1-7
• Publication date: 2012-06-29
• DOI: 10.1016/j.jmb.2012.04.002
• EISSN: 1089-8638
• ISSN: 0022-2836
• Language: English
• Keywords: endoglycosidase; ADCC; glycosylation; Fc receptor; antibody