RNA interference-mediated knockdown of alpha-synuclein protects human dopaminergic neuroblastoma cells from MPP(+) toxicity and reduces dopamine transport.

Journal article

The critical observation in the pathology of Parkinson's disease (PD) is that neurodegeneration is largely restricted to dopaminergic neurons that develop cytoplasmic inclusions called Lewy bodies. These aggregations contain the protein alpha-synuclein. Furthermore, it is becoming apparent that alpha-synuclein expression levels are a major factor in PD pathogenesis. Patients with additional copies of the alpha-synuclein gene develop PD with a severity proportional to levels of alpha-synuclein...

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Authors: Fountaine, T; Wade-Martins, R

• Publication status: Published
• Journal: Journal of neuroscience research
• Volume: 85
• Issue: 2
• Pages: 351-363
• Publication date: 2007-02-01
• DOI: 10.1002/jnr.21125
• EISSN: 1097-4547
• ISSN: 0360-4012
• Language: English
• Keywords: Neurons; Neurotoxins; Humans; RNA Interference; Dopamine; Fluorescent Antibody Technique; Blotting, Western; Neuroblastoma; alpha-Synuclein; Protein Transport; 1-Methyl-4-phenylpyridinium; Dopamine Plasma Membrane Transport Proteins; Transfection; Cell Survival; Reverse Transcriptase Polymerase Chain Reaction; Rotenone; Cell Line, Tumor