Journal article icon

Journal article

Codon optimization reveals critical factors for high level expression of two rare codon genes in Escherichia coli: RNA stability and secondary structure but not tRNA abundance.

Abstract:

Expression patterns in Escherichia coli of two small archaeal proteins with a natural content of about 30% rare codons were analyzed. The proteins, a histone-like protein from Sulfolobus shibatae (Ssh10), and a glutaredoxin-like protein from Methanobacterium thermoautotrophicum (mtGrx), were produced with expression plasmids encoding wild-type genes, codon-optimized synthetic, and GST-fusion genes. These constructs were expressed in BL21 (DE3), its LysS derivative, and modified strains carryi...

Expand abstract
Publication status:
Published

Actions


Access Document


Publisher copy:
10.1016/j.bbrc.2003.11.091

Authors


Journal:
Biochemical and biophysical research communications
Volume:
313
Issue:
1
Pages:
89-96
Publication date:
2004-01-05
DOI:
EISSN:
1090-2104
ISSN:
0006-291X
URN:
uuid:c8e3db56-a493-4be0-968d-471736b74f14
Source identifiers:
108564
Local pid:
pubs:108564

Terms of use


Metrics


Views and Downloads






If you are the owner of this record, you can report an update to it here: Report update to this record

TO TOP