Role of initiating nucleoside triphosphate concentrations in the regulation of influenza virus replication and transcription.

Journal article

The mechanisms regulating the synthesis of mRNA, cRNA, and viral genomic RNA (vRNA) by the influenza A virus RNA-dependent RNA polymerase are not fully understood. Previous studies in our laboratory have shown that virion-derived viral ribonucleoprotein complexes synthesize both mRNA and cRNA in vitro and early in the infection cycle in vivo. Our continued studies showed that de novo synthesis of cRNA in vitro is more sensitive to the concentrations of ATP, CTP, and GTP than capped-primer-dep...

Expand abstract

Authors: Vreede, F; Gifford, H; Brownlee, G

• Publication status: Published
• Journal: Journal of virology
• Volume: 82
• Issue: 14
• Pages: 6902-6910
• Publication date: 2008-07-01
• DOI: 10.1128/jvi.00627-08
• EISSN: 1098-5514
• ISSN: 0022-538X
• Language: English
• Keywords: RNA Replicase; Cell Line; Point Mutation; RNA, Messenger; Transcription, Genetic; Models, Biological; Humans; RNA, Viral; Influenza A virus; Animals; Nucleotides; Cattle; Viral Plaque Assay; Viral Proteins; Virus Replication