Application of ImageJ program to the enumeration of Orientia tsutsugamushi organisms cultured in vitro.

Journal article

The ImageJ program was applied to the enumeration of Orientia tsutsugamushi organisms in cell culture using indirect immunofluorescence assay (IFA). The highest correlation (r=0.984) was observed between manual counting methods and the ImageJ program (MaxEntropy threshold algorithm). This software-based methodology is cheaper, more standardised and better reproducible than a manual-based approach.

Authors: Siritantikorn, S; Jintaworn, S; Noisakran, S; Suputtamongkol, Y; Paris, D

• Publication status: Published
• Journal: Transactions of the Royal Society of Tropical Medicine and Hygiene
• Volume: 106
• Issue: 10
• Pages: 632-635
• Publication date: 2012-10-01
• DOI: 10.1016/j.trstmh.2012.05.004
• EISSN: 1878-3503
• ISSN: 0035-9203
• Language: English
• Keywords: Fluorescent Antibody Technique, Indirect; Humans; Antigens, Bacterial; Scrub Typhus; Cells, Cultured; Bacterial Proteins; Sensitivity and Specificity; Orientia tsutsugamushi; Reproducibility of Results