Rapid ATP-dependent priming of secretory granules precedes Ca(2+)-induced exocytosis in mouse pancreatic B-cells.

Journal article

1. The glucose and ATP dependence of exocytosis were investigated in single mouse pancreatic B-cells by monitoring changes in cell capacitance evoked by voltage-clamp depolarizations, infusion of high [Ca2+]i buffers or photorelease of caged Ca2+ or ATP. 2. In intact B-cells, using the perforated patch whole-cell technique, glucose (5 mM) increased exocytotic responses evoked by membrane depolarization 5-fold over that observed in the absence of the sugar. Increasing the glucose concentration...

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Authors: Eliasson, L; Renström, E; Ding, W; Proks, P; Rorsman, P

• Publication status: Published
• Journal: Journal of physiology
• Volume: 503 ( Pt 2)
• Issue: 2
• Pages: 399-412
• Publication date: 1997-09-01
• DOI: 10.1111/j.1469-7793.1997.399bh.x
• EISSN: 1469-7793
• ISSN: 0022-3751
• Language: English
• Keywords: Islets of Langerhans; Calcium; Membrane Potentials; Electrophysiology; Exocytosis; Glucose; Patch-Clamp Techniques; Animals; Adenosine Triphosphate; Cytoplasmic Granules; Guanosine 5'-O-(3-Thiotriphosphate); Mice