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Rapid ATP-dependent priming of secretory granules precedes Ca(2+)-induced exocytosis in mouse pancreatic B-cells.

Abstract:

1. The glucose and ATP dependence of exocytosis were investigated in single mouse pancreatic B-cells by monitoring changes in cell capacitance evoked by voltage-clamp depolarizations, infusion of high [Ca2+]i buffers or photorelease of caged Ca2+ or ATP. 2. In intact B-cells, using the perforated patch whole-cell technique, glucose (5 mM) increased exocytotic responses evoked by membrane depolarization 5-fold over that observed in the absence of the sugar. Increasing the glucose concentration...

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Published

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Institution:
University of Oxford
Division:
MSD
Department:
RDM
Sub department:
OCDEM
Role:
Author
Journal:
Journal of physiology More from this journal
Volume:
503 ( Pt 2)
Issue:
2
Pages:
399-412
Publication date:
1997-09-01
DOI:
EISSN:
1469-7793
ISSN:
0022-3751
Language:
English
Keywords:
Pubs id:
pubs:26796
UUID:
uuid:c898db00-d271-4304-bb0d-8dc2afa3f5e5
Local pid:
pubs:26796
Source identifiers:
26796
Deposit date:
2012-12-19

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