- Abstract:
-
In proteomics, a digested cell lysate is often too complex for direct comprehensive mass spectrometric analysis. To reduce complexity, several peptide separation techniques have been introduced including very successful two-dimensional liquid chromatography (2D-LC) approaches. Here, we assess the potential of zwitterionic Hydrophilic Interaction Liquid Chromatography (ZIC-HILIC) as a first dimension for the analysis of complex peptide mixtures. We show that ZIC-HILIC separation is dramaticall...
Expand abstract - Publication status:
- Published
- Publisher copy:
- 10.1021/pr060589m
-
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- Journal:
- Journal of proteome research
- Volume:
- 6
- Issue:
- 3
- Pages:
- 937-946
- Publication date:
- 2007-03-05
- DOI:
- EISSN:
-
1535-3907
- ISSN:
-
1535-3893
- URN:
-
uuid:c7f49a7b-5585-45b7-baec-67bf50979d93
- Source identifiers:
-
384608
- Local pid:
- pubs:384608
- Language:
- English
- Keywords:
- Copyright date:
- 2007
Journal article
Evaluation and optimization of ZIC-HILIC-RP as an alternative MudPIT strategy.
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