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Evaluation and optimization of ZIC-HILIC-RP as an alternative MudPIT strategy.

Abstract:

In proteomics, a digested cell lysate is often too complex for direct comprehensive mass spectrometric analysis. To reduce complexity, several peptide separation techniques have been introduced including very successful two-dimensional liquid chromatography (2D-LC) approaches. Here, we assess the potential of zwitterionic Hydrophilic Interaction Liquid Chromatography (ZIC-HILIC) as a first dimension for the analysis of complex peptide mixtures. We show that ZIC-HILIC separation is dramaticall...

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Publication status:
Published

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Publisher copy:
10.1021/pr060589m

Authors


Boersema, PJ More by this author
Divecha, N More by this author
More by this author
Institution:
University of Oxford
Department:
Oxford, MPLS, Chemistry, Physical and Theoretical Chem
Journal:
Journal of proteome research
Volume:
6
Issue:
3
Pages:
937-946
Publication date:
2007-03-05
DOI:
EISSN:
1535-3907
ISSN:
1535-3893
URN:
uuid:c7f49a7b-5585-45b7-baec-67bf50979d93
Source identifiers:
384608
Local pid:
pubs:384608

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