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Coma aberrations in combined two- and three-dimensional STED nanoscopy

Abstract:

Stimulated emission depletion (STED) microscopes, like all super-resolution methods, are sensitive to aberrations. Of particular importance are aberrations that affect the quality of the depletion focus, which requires a point of near-zero intensity surrounded by strong illumination. We present analysis, modelling and experimental measurements that show the effects of coma aberrations on depletion patterns of two-dimensional (2D) and three-dimensional (3D) STED configurations. Specifically, w...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1364/OL.41.003631

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More by this author
Institution:
University of Oxford
Division:
MSD
Department:
Physiology Anatomy & Genetics
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MSD
Department:
Physiology Anatomy & Genetics
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MPLS
Department:
Engineering Science
Role:
Author
G. Harold and Leila Y. Mathers Charitable Foundation More from this funder
Publisher:
Optical Society of America Publisher's website
Journal:
Optics Letters Journal website
Volume:
41
Issue:
15
Pages:
3631-3634
Publication date:
2016-01-01
Acceptance date:
2016-05-27
DOI:
EISSN:
1539-4794
ISSN:
0146-9592
Keywords:
Pubs id:
pubs:627636
UUID:
uuid:c79f5072-f2e2-49fa-b25a-dd19811fe71e
Local pid:
pubs:627636
Source identifiers:
627636
Deposit date:
2016-06-13

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