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Oxygen Partial Pressure Is a Rate-Limiting Parameter for Cell Proliferation in 3D Spheroids Grown in Physioxic Culture Condition

Abstract:

The in situ oxygen partial pressure in normal and tumor tissues is in the range of a few percent. Therefore, when studying cell growth in 3D culture systems, it is essential to consider how the physiological oxygen concentration, rather than the one in the ambient air, influences the proliferation parameters. Here, we investigated the effect of reducing oxygen partial pressure from 21% to 5% on cell proliferation rate and regionalization in a 3D tumor spheroid model. We found that 5% oxygen c...

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Publication status:
Published
Peer review status:
Peer reviewed
Version:
Publisher's version

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Publisher copy:
10.1371/journal.pone.0161239

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Department:
Oxford, MSD, Oncology
Role:
Author
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Funding agency for:
Ducommun, B
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Funding agency for:
Ducommun, B
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Funding agency for:
Ducommun, B
Publisher:
Public Library of Science Publisher's website
Journal:
PLoS One Journal website
Publication date:
2016-08-30
Acceptance date:
2016-08-02
DOI:
EISSN:
1932-6203
ISSN:
1932-6203
Pubs id:
pubs:641523
URN:
uri:c792eca4-2158-4003-b947-caf89c5c8d0d
UUID:
uuid:c792eca4-2158-4003-b947-caf89c5c8d0d
Local pid:
pubs:641523

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