Diffraction-unlimited all-optical imaging and writing with a photochromic GFP.

Journal article

Lens-based optical microscopy failed to discern fluorescent features closer than 200 nm for decades, but the recent breaking of the diffraction resolution barrier by sequentially switching the fluorescence capability of adjacent features on and off is making nanoscale imaging routine. Reported fluorescence nanoscopy variants switch these features either with intense beams at defined positions or randomly, molecule by molecule. Here we demonstrate an optical nanoscopy that records raw data ima...

Expand abstract

Authors: Grotjohann, T; Testa, I; Leutenegger, M; Bock, H; Urban, N

• Publication status: Published
• Journal: Nature
• Volume: 478
• Issue: 7368
• Pages: 204-208
• Publication date: 2011-10-01
• DOI: 10.1038/nature10497
• EISSN: 1476-4687
• ISSN: 0028-0836
• Language: English
• Keywords: Brain; Photobleaching; Dendrites; Optics and Photonics; Animals; Cell Line; Escherichia coli; Nanotechnology; Cell Survival; Microscopy, Fluorescence; Light; Green Fluorescent Proteins; Equipment Reuse