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Inter- and intra-operator variability in the reading of indirect immunofluorescence assays for the serological diagnosis of scrub typhus and murine typhus.

Abstract:

Inter- and intra-observer variation was examined among six microscopists who read 50 scrub typhus (ST) and murine typhus (MT) indirect immunofluorescence assay (IFA) immunoglobulin M (IgM) slides. Inter-observer agreement was moderate (κ = 0.45) for MT and fair (κ = 0.32) for ST, and was significantly correlated with experience (P = 0.03 and P = 0.004, respectively); κ-scores for intra-observer agreement between morning and afternoon readings (range = 0.35-0.86) were not correlated between ye...

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Publication status:
Published

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Publisher copy:
10.4269/ajtmh.12-0325

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Journal:
American journal of tropical medicine and hygiene More from this journal
Volume:
88
Issue:
5
Pages:
932-936
Publication date:
2013-05-01
DOI:
EISSN:
1476-1645
ISSN:
0002-9637
Language:
English
Keywords:
Pubs id:
pubs:387753
UUID:
uuid:c76cc0e0-ce4b-416a-8fcc-0219fa7a57ab
Local pid:
pubs:387753
Source identifiers:
387753
Deposit date:
2014-02-07

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