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Journal article

Robust RNA-based in situ mutation detection delineates colorectal cancer subclonal evolution.

Abstract:

Intra-tumor heterogeneity (ITH) is a major underlying cause of therapy resistance and disease recurrence, and is a read-out of tumor growth. Current genetic ITH analysis methods do not preserve spatial context and may not detect rare subclones. Here, we address these shortfalls by developing and validating BaseScope-a novel mutation-specific RNA in situ hybridization assay. We target common point mutations in the BRAF, KRAS and PIK3CA oncogenes in archival colorectal cancer samples to precise...

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Publication status:
Published
Peer review status:
Peer reviewed

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Authors


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Name:
Medical Research Council
Grant:
MR/M016587/1
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Name:
Chris Rokos Fellowship in Evolution and Cancer
Grant:
Grant Number Example
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Name:
Wellcome Trust
Grant:
MR/M016587/1
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Name:
European Research Council
Grant:
INSTITUMUT
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Name:
Cancer Research UK
Grant:
C52506/A22909
Publisher:
Nature Publishing Group
Journal:
Nature Communications More from this journal
Volume:
8
Issue:
1
Pages:
1998
Publication date:
2017-12-01
Acceptance date:
2017-11-17
DOI:
EISSN:
2041-1723
Pmid:
29222441
Language:
English
Keywords:
Pubs id:
pubs:810366
UUID:
uuid:c760f0fb-770e-4d5f-a26d-16f41697dffc
Local pid:
pubs:810366
Source identifiers:
810366
Deposit date:
2018-01-24

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