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Journal article

Quantitative 3D structured illumination microscopy of nuclear structures

Abstract:

3D structured illumination microscopy (3D-SIM) is the super-resolution technique of choice for multicolor volumetric imaging. Here we provide a validated sample preparation protocol for labeling nuclei of cultured mammalian cells, image acquisition and registration practices, and downstream image analysis of nuclear structures and epigenetic marks. Using immunostaining and replication labeling combined with image segmentation, centroid mapping and nearest-neighbor analyses in open-source envi...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1038/nprot.2017.020

Authors


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Institution:
University of Oxford
Division:
MSD
Department:
Biochemistry
Role:
Author
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Institution:
University of Oxford
Division:
MSD
Department:
Biochemistry
Role:
Author
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Funding agency for:
Markaki, Y
Grant:
JP26292169
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Grant:
Nanosystems Initiative Munich
Publisher:
Nature Publishing Group Publisher's website
Journal:
Nature Protocols Journal website
Volume:
12
Issue:
5
Pages:
1011–1028
Publication date:
2017-04-13
Acceptance date:
2017-02-16
DOI:
EISSN:
1750-2799
ISSN:
1754-2189
Source identifiers:
691282
Language:
English
Keywords:
Pubs id:
pubs:691282
UUID:
uuid:c7290bbc-d2c7-4143-9fc1-10a888cddc06
Local pid:
pubs:691282
Deposit date:
2017-04-27

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