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Journal article

Quantitative 3D structured illumination microscopy of nuclear structures

Abstract:

3D structured illumination microscopy (3D-SIM) is the super-resolution technique of choice for multicolor volumetric imaging. Here we provide a validated sample preparation protocol for labeling nuclei of cultured mammalian cells, image acquisition and registration practices, and downstream image analysis of nuclear structures and epigenetic marks. Using immunostaining and replication labeling combined with image segmentation, centroid mapping and nearest-neighbor analyses in open-source envi...

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Publication status:
Published
Peer review status:
Peer reviewed
Version:
Accepted manuscript

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Publisher copy:
10.1038/nprot.2017.020

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Department:
Oxford, MSD, Biochemistry
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Department:
Oxford, MSD, Biochemistry
Chitiashvili, T More by this author
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Publisher:
Nature Publishing Group Publisher's website
Journal:
Nature Protocols Journal website
Volume:
12
Issue:
5
Pages:
1011–1028
Publication date:
2017-04-13
Acceptance date:
2017-02-16
DOI:
EISSN:
1750-2799
ISSN:
1754-2189
Pubs id:
pubs:691282
URN:
uri:c7290bbc-d2c7-4143-9fc1-10a888cddc06
UUID:
uuid:c7290bbc-d2c7-4143-9fc1-10a888cddc06
Local pid:
pubs:691282

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