Fine mapping of the human MHC class II region within chromosome band 6p21 and evaluation of probe ordering using interphase fluorescence in situ hybridization.

Journal article

Eight previously well-characterized and mapped probes derived from the human major histocompatibility complex (MHC) class II region were used to investigate the advantages and limitations of fluorescence in situ hybridization (FISH) techniques for fine mapping. The class II region of the MHC was localized within subband 6p21.31 by in situ hybridization on metaphase chromosomes that were banded by immunofluorescence staining with an antibody against 5-bromodeoxyuridine (BrdU). Ordering of prob...

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Authors: Senger, G; Ragoussis, I; Trowsdale, J; Sheer, D

• Publication status: Published
• Journal: Cytogenetics and cell genetics
• Volume: 64
• Issue: 1
• Pages: 49-53
• Publication date: 1993-01-01
• DOI: 10.1159/000133559
• EISSN: 1424-859X
• ISSN: 0301-0171
• Language: English
• Keywords: Chromosomes, Human, Pair 6; Genes, MHC Class II; In Situ Hybridization, Fluorescence; DNA Probes; Humans; Interphase; Cosmids; Lymphocytes; Chromosome Mapping