Genome-wide discovery and verification of novel structured RNAs in Plasmodium falciparum.

Journal article

We undertook a genome-wide search for novel noncoding RNAs (ncRNA) in the malaria parasite Plasmodium falciparum. We used the RNAz program to predict structures in the noncoding regions of the P. falciparum 3D7 genome that were conserved with at least one of seven other Plasmodium spp. genome sequences. By using Northern blot analysis for 76 high-scoring predictions and microarray analysis for the majority of candidates, we have verified the expression of 33 novel ncRNA transcripts including ...

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Authors: Mourier, T; Carret, C; Kyes, S; Christodoulou, Z; Gardner, P

• Publication status: Published
• Journal: Genome research
• Volume: 18
• Issue: 2
• Pages: 281-292
• Publication date: 2008-02-01
• DOI: 10.1101/gr.6836108
• EISSN: 1549-5469
• ISSN: 1088-9051
• Language: English
• Keywords: Phylogeny; Base Sequence; Genome, Protozoan; Computational Biology; Sequence Analysis, DNA; Sequence Alignment; Microarray Analysis; Plasmodium falciparum; Species Specificity; Molecular Sequence Data; Animals; Models, Genetic; Base Pairing; Evolution, Molecular; Conserved Sequence; Chromosome Mapping; Blotting, Northern; RNA, Untranslated