Human nuclear Dicer restricts the deleterious accumulation of endogenous double-stranded RNA.

Journal article

Dicer is a central enzymatic player in RNA-interference pathways that acts to regulate gene expression in nearly all eukaryotes. Although the cytoplasmic function of Dicer is well documented in mammals, its nuclear function remains obscure. Here we show that Dicer is present in both the nucleus and cytoplasm, and its nuclear levels are tightly regulated. Dicer interacts with RNA polymerase II (Pol II) at actively transcribed gene loci. Loss of Dicer causes the appearance of endogenous double-...

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Authors: White, E; Schlackow, M; Kamieniarz-Gdula, K; Proudfoot, N; Gullerova, M

• Publication status: Published
• Journal: Nature structural and molecular biology
• Volume: 21
• Issue: 6
• Pages: 552-559
• Publication date: 2014-06-01
• DOI: 10.1038/nsmb.2827
• EISSN: 1545-9985
• ISSN: 1545-9993
• Language: English
• Keywords: Apoptosis; Cell Nucleus; Chromatin; Ribonuclease III; HEK293 Cells; Humans; RNA, Double-Stranded; Interferons; Fluorescent Antibody Technique; RNA Polymerase II; Signal Transduction; DEAD-box RNA Helicases