DNA restriction-fragment variation in the gene family encoding high molecular weight (HMW) glutenin subunits of wheat.

Journal article

Restriction enzyme digests of DNA from nullisomic-tetrasomic and intervarietal chromosome substitution lines of wheat were probed with a high molecular weight (HMW) glutenin cDNA. Three restriction endonucleases were used to investigate restriction-fragment differences among five wheat varieties. The results suggest that the hybridizing fragments contain single gene copies and permit the identification of the subunit encoded by each gene. Restriction-fragment variation associated with previou...

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Authors: Harberd, N; Bartels, D; Thompson, R

• Publication status: Published
• Publisher: Kluwer Academic Publishers-Plenum Publishers
• Journal: Biochemical genetics
• Volume: 24
• Issue: 7-8
• Pages: 579-596
• Publication date: 1986-08-01
• DOI: 10.1007/bf00504336
• EISSN: 1573-4927
• ISSN: 0006-2928
• Language: English
• Keywords: Alleles; Triticum; Glutens; Plants; Genes; DNA Restriction Enzymes; Macromolecular Substances; Genetic Variation; Chromosome Mapping; Molecular Weight; Amino Acid Sequence; Nucleic Acid Hybridization