The DNA-remodelling activity of DnaD is the sum of oligomerization and DNA-binding activities on separate domains.

Journal article

The Bacillus subtilis DnaD protein is an essential protein that has been implicated in the primosomal step of DNA replication, and recently in global DNA remodelling. Here we show that DnaD consists of two domains with distinct activities; an N-terminal domain (Nd) with oligomerization activity, and a C-terminal domain (Cd) with DNA-binding activity and a second DNA-induced oligomerization activity. Although Cd can bind to DNA and form large nucleoprotein complexes, it does not exhibit global...

Expand abstract

Authors: Carneiro, M; Zhang, W; Ioannou, C; Scott, D; Allen, S

• Publication status: Published
• Journal: Molecular microbiology
• Volume: 60
• Issue: 4
• Pages: 917-924
• Publication date: 2006-05-01
• DOI: 10.1111/j.1365-2958.2006.05152.x
• EISSN: 1365-2958
• ISSN: 0950-382X
• Language: English
• Keywords: DNA Replication; Plasmids; Bacillus subtilis; Amino Acid Motifs; DNA-Binding Proteins; Bacterial Proteins; Protein Structure, Tertiary; DNA, Superhelical