Detection of nascent RNA transcripts by fluorescence in situ hybridization.

Journal article

The development of cellular diversity within any organism depends on the timely and correct expression of differing subsets of genes within each tissue type. Many techniques exist which allow a global, average analysis of RNA expression; however, RNA-FISH permits the sensitive detection of specific transcripts within individual cells while preserving the cellular morphology. The technique can provide insight into the spatial and temporal organization of gene transcription as well the relation...

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Authors: Brown, J; Buckle, V

• Journal: Methods in molecular biology (Clifton, N.J.)
• Volume: 659
• Pages: 33-50
• Publication date: 2010-01-01
• DOI: 10.1007/978-1-60761-789-1_3
• EISSN: 1940-6029
• ISSN: 1064-3745
• Language: English
• Keywords: In Situ Hybridization, Fluorescence; Oligonucleotide Probes; Cell Separation; DNA; Animals; Cell Culture Techniques; RNA, Messenger; Blood Cells; Humans