Data registration and selective single-molecule analysis using multi-parameter fluorescence detection.

Journal article

A general strategy to identify and quantify sample molecules in dilute solution employing a new spectroscopic method for data registration and specific burst analysis denoted as multi-parameter fluorescence detection (MFD) was recently developed. While keeping the experimental advantage of monitoring single molecules diffusing through the microscopic open volume element of a confocal epi-illuminated set-up as in experiments of fluorescence correlation spectroscopy, MFD uses pulsed excitation ...

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Authors: Eggeling, C; Berger, S; Brand, L; Fries, JR; Schaffer, J

• Publication status: Published
• Journal: Journal of biotechnology
• Volume: 86
• Issue: 3
• Pages: 163-180
• Publication date: 2001-04-01
• DOI: 10.1016/s0168-1656(00)00412-0
• EISSN: 1873-4863
• ISSN: 0168-1656
• Language: English
• Keywords: Oligonucleotides; Luminescent Proteins; Fluorescent Dyes; Rhodamine 123; Models, Chemical; Time Factors; Spectrometry, Fluorescence; Image Processing, Computer-Assisted; Green Fluorescent Proteins