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Assignment of protein NMR spectra using heteronuclear NMR: a tutorial

Abstract:

The assignment of resonances in the complex nuclear magnetic resonance (NMR) spectrum of a protein is the first step in any NMR study of protein structure, function or dynamics. This chapter aims to provide a tutorial on protein NMR resonance assignment. Two approaches to the assignment are commonly used: the triple resonance methodology, which uses a suite of three-dimensional (3D) 13C/15N/1H NMR experiments, relies on through-bond 1J and 2J intra- and interresidue spin–spin couplings that a...

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Publication status:
Published
Peer review status:
Peer reviewed

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Files:
Publisher copy:
10.1007/978-1-4899-7621-5_1
Publication website:
https://link.springer.com/book/10.1007/978-1-4899-7621-5

Authors


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Institution:
University of Oxford
Division:
MSD
Department:
Biochemistry
Role:
Author

Contributors

Role:
Editor
Publisher:
Springer US Publisher's website
Pages:
1-42
Series:
Biological Magnetic Resonance
Series number:
32
Host title:
Protein NMR: Modern Techniques and Biomedical Applications
Publication date:
2015-08-25
DOI:
EISBN:
978-1-4899-7621-5
ISSN:
0192-6020
Source identifiers:
599050
ISBN:
978-1-4899-7620-8
Language:
English
Keywords:
Subtype:
Chapter
Pubs id:
pubs:599050
UUID:
uuid:c5b50a2d-d1de-4e28-9e10-12d93877c364
Local pid:
pubs:599050
Deposit date:
2016-02-05

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