Investigation into techniques to isolate and develop human germ cells in vitro

Thesis

Premature ovarian insufficiency (POI) occurs in 1% of women younger than 40 years of age. The manifestation of this condition varies considerably ranging from ovaries devoid of follicles (afollicular POI) to ovaries containing follicles at various stages of development (follicular POI). No methods exist in mainstream healthcare to restore ovarian function for POI patients and ≥70% of POI cases are idiopathic. The Reaggregated Ovary (RO) method, as established for mice, enables investigatio...

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Authors: Papadopoulou, E

• Type of award: DPhil
• Level of award: Doctoral
• Awarding institution: University of Oxford
• Language: English
• Keywords: Remnants; DEAD-box helicase 4 (DDX4); Follicle depletion; Follicle dysfunction; Effect of age on human ovarian tissue dissection; Flow Cytometry; Follicle development in vitro; Fertility Preservation; Afollicular; Premature Ovarian Insufficiency (POI); Human ovarian tissue enzymatic digestion; Cortex; Human ovarian tissue dissection; Reaggregated Ovaries (RO); Follicular; Human dermal fibroblasts
• Subjects: Fertility