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A ratiometric method of autofluorescence correction used for the quantification of Evans blue dye fluorescence in rabbit arterial tissues.

Abstract:

Evans blue dye (EBD) conjugates with albumin in the circulation and is frequently used to measure vascular protein leakage. The fluorescence of the dye from tissue sections can be used to measure its uptake at very specific anatomical locations, but problems arise with dye quantification because tissue components also fluoresce; so-called autofluorescence. We have measured uptake of EBD by blood vessel walls at various points around the aorto-renal branch of rabbits. High resolution, digitise...

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Publication status:
Published

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Publisher copy:
10.1113/eph8702332

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Institution:
University of Oxford
Department:
Oxford, MSD, NDORMS
Journal:
Experimental physiology
Volume:
87
Issue:
2
Pages:
163-170
Publication date:
2002-03-05
DOI:
EISSN:
1469-445X
ISSN:
0958-0670
URN:
uuid:c4b66de5-49a6-42b2-84a2-3c41ee854b6f
Source identifiers:
227077
Local pid:
pubs:227077

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