The effect of aglycosylation on the immunogenicity of a humanized therapeutic CD3 monoclonal antibody.

Journal article

The factors affecting the immunogenicity of a humanized gamma 1 CD3 monoclonal antibody (mAb) were investigated in transgenic mice that express the human CD3 antigen epsilon polypeptide (the mAb target antigen). Two derivatives of the mAb were employed, one with a normal, glycosylated Fc region (gamma 1 CD3 mAb), and the other with an aglycosylated Fc region (aglycosyl gamma 1 CD3 mAb). Comparisons of the antiglobulin responses elicited by the two derivatives in transgenic and nontransgenic m...

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Authors: Routledge, E; Falconer, M; Pope, H; Lloyd, I; Waldmann, H

• Publication status: Published
• Journal: Transplantation
• Volume: 60
• Issue: 8
• Pages: 847-853
• Publication date: 1995-10-01
• EISSN: 1534-6080
• ISSN: 0041-1337
• Language: English
• Keywords: Humans; Immunoglobulin Fc Fragments; Drug Design; Antibodies, Monoclonal; Antigens, CD3; Mice; Glycosylation; Animals; Mice, Transgenic; Immunity; Mice, Inbred BALB C