Direct wavefront sensing in adaptive microscopy

Thesis

Specimen-induced aberrations are frequently encountered in high resolution microscopy, particularly when high numerical aperture lenses are used to image deep into biological specimens. These aberrations distort the focal spot causing reduction in resolution, signal level and contrast. Aberrations arise due to refractive index variations in the specimen. Adaptive optics has been used in microscopes to correct for these effects and restore optimum imaging performance. An adaptive element, s...

Expand abstract

Authors: Rahman, S; Saad Azmeen-ur-Rahman

• Publication date: 2012
• Type of award: MSc
• Level of award: Masters
• Awarding institution: University of Oxford
• Language: English
• Keywords: Shack-Hartmann; specimen-induced aberration; microscopy; backscattered light; Direct wavefront sensing
• Subjects: Optics,electromagnetic theory (mathematics); Electronics; Electrical engineering; Optoelectronics; Medical Sciences; Biomedical engineering; Sensors