Epithelial cells bearing class II molecules stimulate allogeneic human colonic intraepithelial lymphocytes.

Journal article

HLA-DR+ gut epithelial cells may present antigen to intraepithelial lymphocytes (IEL). This study aimed to isolate an IEL population from the human colon to activate CD3 + IEL by a human colonic epithelial cell line (HT-29), bearing different concentrations of class II antigen (HLA-DR). IEL were isolated by a mechanical method from six patients with ulcerative colitis (UC) and from 14 control patients. IEL were cocultured with HT-29 which had been induced to express class II molecules by gamm...

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Authors: Hoang, P; Crotty, B; Dalton, H; Jewell, D

• Publication status: Published
• Publisher: BMJ Publishing Group
• Journal: Gut
• Volume: 33
• Issue: 8
• Pages: 1089-1093
• Publication date: 1992-08-01
• DOI: 10.1136/gut.33.8.1089
• EISSN: 1468-3288
• ISSN: 0017-5749
• Language: English
• Keywords: Female; Humans; Tumor Cells, Cultured; Colitis, Ulcerative; Lymphocytes; CD4-CD8 Ratio; HLA-DR Antigens; Colon; Middle Aged; Adult; Epithelium; Interferon-gamma; Aged; Antigen-Presenting Cells; Antigens, CD; Male; Lymphocyte Activation