Collision cross sections of proteins and their complexes: a calibration framework and database for gas-phase structural biology.

Journal article

Collision cross sections in both helium and nitrogen gases were measured directly using a drift cell with RF ion confinement inserted within a quadrupole/ion mobility/time-of-flight hybrid mass spectrometer (Waters Synapt HDMS, Manchester, U.K.). Collision cross sections for a large set of denatured peptide, denatured protein, native-like protein, and native-like protein complex ions are reported here, forming a database of collision cross sections that spans over 2 orders of magnitude. The a...

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Authors: Bush, M; Hall, Z; Giles, K; Hoyes, J; Robinson, C

• Publication status: Published
• Journal: Analytical chemistry
• Volume: 82
• Issue: 22
• Pages: 9557-9565
• Publication date: 2010-11-01
• DOI: 10.1021/ac1022953
• EISSN: 1520-6882
• ISSN: 0003-2700
• Language: English
• Keywords: Pressure; Protein Denaturation; Helium; Motion; Nitrogen; Databases, Protein; Proteins; Calibration; Alcohol Dehydrogenase; Mass Spectrometry; Radio Waves