Characterization of the MCRred2 form of methyl-coenzyme M reductase: a pulse EPR and ENDOR study.
Methyl-coenzyme M reductase (MCR), which catalyses the reduction of methyl-coenzyme M (CH(3)-S-CoM) with coenzyme B (H-S-CoB) to CH(4) and CoM-S-S-CoB, contains the nickel porphinoid F430 as prosthetic group. The active enzyme exhibits the Ni(I)-derived axial EPR signal MCR(red1) both in the absence and presence of the substrates. When the enzyme is competitively inhibited by coenzyme M (HS-CoM) the MCR(red1) signal is partially converted into the rhombic EPR signal MCR(red2). To obtain deepe...Expand abstract
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- Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry
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