Characterization of Na/Ca exchange in plasmalemmal vesicles from zona fasciculata cells of the bovine adrenal gland.

Journal article

The presence of an Na/Ca exchange system in fasciculata cells of the bovine adrenal gland was tested using isolated plasmalemmal vesicles. In the presence of an outwardly Na(+) gradient, Ca(2+) uptake was about 2-fold higher than in K(+) condition. Li(+) did not substitute for Na(+) and 5 mM Ni(2+) inhibited Ca(2+) uptake. Ca(2+) efflux from Ca(2+)-loaded vesicles was Na(+)-stimulated and Ni(2+)-inhibited. The saturable part of Na(+)-dependent Ca(2+) uptake displayed Michaelis-Menten kinetics...

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Authors: Brunold, C; Guyot, A; Noble, D; Rougier, O; Bilbaut, A

• Publication status: Published
• Journal: Biochimica et biophysica acta
• Volume: 1466
• Issue: 1-2
• Pages: 361-371
• Publication date: 2000-06-01
• DOI: 10.1016/s0005-2736(00)00202-9
• ISSN: 0006-3002
• Language: English
• Keywords: Calcium; Sodium; Cell Membrane; Adrenal Glands; Zona Fasciculata; Sodium-Calcium Exchanger; Animals; Cattle