Femtosecond near-infrared laser pulse induced strand breaks in mammalian cells.

Journal article

Abstract:: Multiphoton laser scanning microscopy (MPLSM) is based on non-resonant simultaneous absorption of two or three near infrared (NIR) photons by a fluorophore in the subfemtoliter focal volume of a high numerical aperture (N.A. 1.3) objective. The higher penetration depth of NIR radiation enables optical sectioning across thick biological specimens and because of the absence of efficient single photon absorbers in the NIR spectral region of 700 to 1200 nm there is hardly any out-of-focus photoda...
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Institution:

University of Oxford

Division:

MSD

Department:

NDORMS

Role:

Author

Role:

Author

Journal:: Cellular and molecular biology (Noisy-le-Grand, France) More from this journal
Volume:: 47 Online Pub
Pages:: OL131-OL134
Publication date:: 2001-01-01
EISSN:: 1165-158X
ISSN:: 0145-5680

Language:: English
Keywords:: Macropodidae

DNA Damage

Lasers

Apoptosis

Animals

In Situ Nick-End Labeling

Infrared Rays

DNA Fragmentation

Microscopy, Confocal

Cell Line
Pubs id:: pubs:108389
UUID:: uuid:c4142507-a77c-4b56-af09-181e6dd0c4b8
Local pid:: pubs:108389
Source identifiers:: 108389
Deposit date:: 2012-12-19

Licence:: Terms and Conditions of Use for Oxford University Research Archive

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