Expression of prolyl-hydroxylases PHD-1, 2 and 3 and of the asparagine hydroxylase FIH in non-small cell lung cancer relates to an activated HIF pathway.

Journal article

The oxygen sensitive prolyl-hydroxylase domain enzymes (PHDs) and the asparagines hydroxylase (FIH, factor inhibiting HIF) regulate the transcriptional activity of HIFs. We assessed the expression of these enzymes in a series of 73 non-small cell lung carcinomas (NSCLC). A direct association of PHDs with FIH and of the PHDs/FIH with HIFs expression was noted. Thirty three of 73 cases had high HIF/PHD expression, predicting intense HIF activity; 19/73 cases had low HIF and high PHD expression mimicking the normal bronchial pattern; and 18/73 cases had low HIF/PHD (inactive HIF pathway). High lactate dehydrogenase LDH5 expression was noted in cases with high HIF/PHD phenotype. The value of such a classification in defining different metabolic phenotypes of NSCLC deserves further evaluation.

Authors: Giatromanolaki, A; Koukourakis, M; Pezzella, F; Turley, H; Sivridis, E

• Publication status: Published
• Journal: Cancer letters
• Volume: 262
• Issue: 1
• Pages: 87-93
• Publication date: 2008-04-01
• DOI: 10.1016/j.canlet.2007.11.041
• EISSN: 1872-7980
• ISSN: 0304-3835
• Language: English
• Keywords: Carcinoma, Non-Small-Cell Lung; Asparagine; Mixed Function Oxygenases; Hypoxia-Inducible Factor 1; Procollagen-Proline Dioxygenase; Lung Neoplasms; Repressor Proteins; Humans; Transcriptional Activation