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Simultaneous identification of species and molecular forms of the Anopheles gambiae complex by PCR-RFLP.

Abstract:
For differential identification of sibling species in the Anopheles gambiae Giles complex (Diptera: Culicidae), including simultaneous separation of M and S molecular forms within An. gambiae Giles sensu stricto, we describe a PCR-RFLP method. This procedure is more efficient, faster and cheaper than those used before, so is recommended for large-scale processing of field-collected larval and adult specimens to be identified in malaria vector studies.

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Institution:
University of Oxford
Department:
Oxford, MSD, Clinical Medicine, Thailand/Laos MOP
Role:
Author
Journal:
Medical and veterinary entomology
Volume:
16
Issue:
4
Pages:
461-464
Publication date:
2002-12-05
DOI:
EISSN:
1365-2915
ISSN:
0269-283X
URN:
uuid:c3e94535-f85f-4bdf-ab7c-c51020cd1a49
Source identifiers:
258521
Local pid:
pubs:258521

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